Journal: BMC Genomics

Article Title: Single-cell RNA-seq mapping of chicken peripheral blood leukocytes

doi: 10.1186/s12864-024-10044-4

Figure Lengend Snippet: Marker genes used for primary annotation of cell types among chicken leukocytes

Article Snippet: Cells were suspended in PBS supplemented with 2% foetal bovine serum (Gibco® #10,082,147, ThermoFisher Scientific) and 1 mM EDTA and the number of thrombocytes in the samples were reduced by immunomagnetic cell separation using the EasySep PE Positive Selection Kit II (#17,684, StemCell Technologies) and PE-conjugated mouse monoclonal antibody to chicken CD41/61 (clone 11C3, #AM05550PE-N, OriGene) according to the StemCell Technologies depletion protocol no. 28,898 as previously described for chicken leukocytes [ ].

Techniques: Marker, Expressing, Binding Assay

Journal: Developmental and Comparative Immunology

Article Title: Regulation and function of macrophage colony-stimulating factor (CSF1) in the chicken immune system

doi: 10.1016/j.dci.2019.103586

Figure Lengend Snippet: List of primary and secondary antibodies used for flow cytometry and immunohistochemistry.

Article Snippet: Mouse anti-CD41/61/clone 11C3 , Integrin CD41/61 , IgG1 , Bio-Rad , 0.8.

Techniques: Flow Cytometry, Immunohistochemistry, Concentration Assay, Control

Journal: Developmental and Comparative Immunology

Article Title: Regulation and function of macrophage colony-stimulating factor (CSF1) in the chicken immune system

doi: 10.1016/j.dci.2019.103586

Figure Lengend Snippet: List of primary and secondary antibodies used for flow cytometry and immunohistochemistry.

Article Snippet: Mouse anti-CD41/61/clone 11C3 , Integrin CD41/61 , IgG1 , Bio-Rad , 0.8.

Techniques: Flow Cytometry, Immunohistochemistry, Concentration Assay, Control

Journal: Developmental and Comparative Immunology

Article Title: Regulation and function of macrophage colony-stimulating factor (CSF1) in the chicken immune system

doi: 10.1016/j.dci.2019.103586

Figure Lengend Snippet: Neutralising effect of mAbs to CSF1. (A) Chicken CSF1 was titrated out on Ba/F3 cells stably transfected with CSF1R (Ba/F3-CSFR) to determine a suitable concentration for neutralising assay. 400 ng/ml was chosen. (B) Ba/F3-CSFR were cultured with CSF1, CSF1 pre-incubated with either ROS-AV181 or 183, mouse IgG1 control or without CSF1 for 72 h before performing MTT assays. Cells cultured with CSF1 or CSF1 pre-incubated with ROS-AV181 or isotype control proliferated. Cells cultured with CSF1 pre-incubated with ROS-AV183 were dead when >2.5 μg/ml of antibody was used. Assays were carried out in triplicate and representative data from three independent experiments are shown. (C) Chicken bone marrow cells were cultured with CSF1 pre-incubated with mAbs, CSF1 only or without CSF1. Original magnification objective lens 10X.

Article Snippet: Mouse anti-CD41/61/clone 11C3 , Integrin CD41/61 , IgG1 , Bio-Rad , 0.8.

Techniques: Stable Transfection, Transfection, Concentration Assay, Cell Culture, Incubation, Control

Journal: Developmental and Comparative Immunology

Article Title: Regulation and function of macrophage colony-stimulating factor (CSF1) in the chicken immune system

doi: 10.1016/j.dci.2019.103586

Figure Lengend Snippet: The effect of anti-CSF1 treatment on lymphoid organs in hatchling birds. CSF1R- eGFP reporter (MacGreen) birds received anti-CSF1 (ROS-AV183) or mouse IgG1 control antibody subcutaneously for four consecutive days from day of hatch and were sacrificed on day 7. (A–C) Mean fluorescence intensity (MFI) and surface area between the two groups obtained using Zen blue 2012 software, showing mean values of spleen, cecal tonsil and bursa. Error bars represent standard error of the mean. Significance was indicated by *P < 0.05 using a Mann–Whitney test. (D–F) Representative whole mount images of lymphoid tissues spleen, cecal tonsil and bursa obtained using Zeiss Axio Cam HR microscope, scale bar = 1 mm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Mouse anti-CD41/61/clone 11C3 , Integrin CD41/61 , IgG1 , Bio-Rad , 0.8.

Techniques: Control, Fluorescence, Software, MANN-WHITNEY, Microscopy

Journal: Developmental and Comparative Immunology

Article Title: Regulation and function of macrophage colony-stimulating factor (CSF1) in the chicken immune system

doi: 10.1016/j.dci.2019.103586

Figure Lengend Snippet: The effect of anti-CSF1 treatment on blood leukocytes and splenocytes. Blood leukocytes and splenocytes were isolated, stained with KUL01, anti-MHC class II and anti-Bu-1 and analysed by flow cytometry. (A–C) The profiles of peripheral blood cells from CSF1R -eGFP birds treated with anti-CSF1 (n = 8) or with mouse IgG1 isotype control antibody (n = 6). Significance is indicated by * P < 0.05 using a Mann–Whitney test. (D–F) The profiles of spleen cell suspension from CSF1R -eGFP birds treated with anti-CSF1 (n = 9) or an isotype control antibody (n = 8). Significance is indicated by *** P < 0.001 using a Mann–Whitney test.

Article Snippet: Mouse anti-CD41/61/clone 11C3 , Integrin CD41/61 , IgG1 , Bio-Rad , 0.8.

Techniques: Isolation, Staining, Flow Cytometry, Control, MANN-WHITNEY, Suspension

Journal: Developmental and Comparative Immunology

Article Title: Regulation and function of macrophage colony-stimulating factor (CSF1) in the chicken immune system

doi: 10.1016/j.dci.2019.103586

Figure Lengend Snippet: The effect of anti-CSF1 treatment on TIM4 + Kupffer cells. (A) Confocal images of Kupffer cells as detected by anti-chicken TIM4 mAb (red) staining in liver tissue (n = 8/9) using LSM 710 inverted microscope. Representative image from three birds from each treatment. Green indicates CSF1R transgene expression, red indicates TIM4 + cells and blue denotes nuclei. Scale bar = 20 μm. (B) The numbers of TIM4 + Kupffer cells per image were quantified using ImageJ from 5 different images from 5 different regions of liver sample per bird per treatment at 40 × magnification. The graph indicates the average count of TIM4 + cells (red channel) per image with standard deviation between the mouse IgG1 and anti-CSF1 treated group. **** indicates significance at P < 0.0001 using a Mann–Whitney test. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Mouse anti-CD41/61/clone 11C3 , Integrin CD41/61 , IgG1 , Bio-Rad , 0.8.

Techniques: Staining, Inverted Microscopy, Expressing, Standard Deviation, MANN-WHITNEY

Journal: Developmental and Comparative Immunology

Article Title: Regulation and function of macrophage colony-stimulating factor (CSF1) in the chicken immune system

doi: 10.1016/j.dci.2019.103586

Figure Lengend Snippet: The effect of anti-CSF1 treatment on bone. (A) Comparison of bone volume and trabecular thickness, number and separation by microCT scanning of femur bones between chicks treated with mouse IgG1 or anti-CSF1 (N = 7/8). Significance is indicated by *P < 0.05 , using a Mann–Whitney test. (B) Representative 3D images from birds treated with mouse IgG1 and anti-CSF1 antibody obtained using CTVox programme. Scale bar = 1.0 mm. (C) Comparison of TRAP + osteoclasts (black arrows) in the bone between mouse IgG1 and anti-CSF1 treated groups. Significance is indicated by ***P < 0.005, using a Mann–Whitney test. (D) Representative TRAP staining images of mouse IgG1 and anti-CSF1 treated birds. Scale bar = 50 μm.

Article Snippet: Mouse anti-CD41/61/clone 11C3 , Integrin CD41/61 , IgG1 , Bio-Rad , 0.8.

Techniques: Comparison, MANN-WHITNEY, Staining